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Best practices in phlebotomy - WHO Guidelines on Drawing Blood

Author: knightzhao

Aug. 25, 2025

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Best practices in phlebotomy - WHO Guidelines on Drawing Blood

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Decoding the Colour Codes of Evacuated Blood Collection Tubes

Invented by Joseph Kleiner and Becton Dickinson in , an evacuated blood collection tube is a sterile plastic or glass test tube that is used to collect blood specimens. It has a coloured rubber stopper that creates a vacuum seal inside the tube, enabling a predetermined volume of liquid to be drawn. Blood collection tubes may contain additives to stabilize and preserve the blood sample prior to analytical testing.

Every evacuated blood collection tube is covered with a colour-coded plastic or rubber cap. The colour helps indicate the type of additives in the tube. Additives include chemical substances like anticoagulants that preserve the blood for processing in a laboratory. Some tubes also contain a gel that has a density between that of blood cells and serum. When these gel-containing tubes are centrifuged, the blood cells sink to the bottom and the serum – separated by a layer of the gel – accumulates on top. Evacuated blood collection tubes not containing gel or a clotting agent, on the other hand, are used when plasma, instead of serum, is required for lab analysis.

Every additive has a specific function and is selected as per the laboratory test to be performed. The colour indicators on the tubes help the phlebotomist identify the tube in which the blood should be drawn in accordance with the recommended test. Since blood collection tubes and tests are connected, using the wrong colour tube may render the blood sample unusable for the intended test.

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The Colour Code

There is a standard protocol to the colour code on blood collection tubes that is universally applied across the medical field. Here is a comprehensive table that sheds light on the colour codes, the additives they indicate, and what they are used for.

There are blood collection tubes for more specific purposes; however, the aforementioned are most commonly used. Safe, convenient, and easy to use, evacuated blood tubes offer several benefits such as facilitating multi-tube draws, while lowering the odds of hemolysis. Consequently, they are widely used in phlebotomy in both developed and developing countries across the world. HMD, a renowned name in the healthcare industry, offers Vaku-8 evacuated tubes that help in collecting blood directly into the tube during venepuncture procedure. The evacuated tubes protect the phlebotomist from the chances of risky blood exposure, thereby ensuring their safety.

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